Journal: bioRxiv
Article Title: Integrated Multiomics Links Metabolic and Inflammatory Remodeling to Arterial Stiffness After the 4,486-km Trans Europe Footrace
doi: 10.64898/2026.03.12.711477
Figure Lengend Snippet: (A) Quantification of reactive oxygen species (ROS) production in Human Aortic Endothelial Cells (HAECs) following incubation with plasma collected Pre- and Post-TEFR. (B) Quantification of nitric oxide (NO) production in HAECs exposed to Pre- and Post-TEFR plasma. Densitometric analysis of eNOS phosphorylation at (C) Ser1177 and (D) Thr495, and (E) total eNOS protein abundance in HAECs exposed to participant plasma. (F) Ex vivo aortic elastic modulus (stiffness) of naïve murine thoracic aortic rings following incubation with Pre- and Post-TEFR plasma. (G) Ex vivo aortic elastic modulus of murine aortic rings incubated with Post-TEFR plasma in the presence or absence of the superoxide dismutase mimetic TEMPOL. (H) In vivo central pulse wave velocity (cPWV) measured in TEFR participants before (Pre) and after (Post) the 4,500-km race. Data are presented as mean ± SEM. Significance determined by paired t-test (A-F, H) or paired t-test comparing vehicle vs. TEMPOL (G).
Article Snippet: Human aortic endothelial cells (HAECs, PromoCell; used after 4–6 passages.
Techniques: Incubation, Clinical Proteomics, Phospho-proteomics, Quantitative Proteomics, Ex Vivo, In Vivo